A team of researchers from the University of Missouri, Kansas State University, and Genus plc have bred pigs that are not harmed by the Porcine Reproductive and Respiratory Syndrome virus, MU announced Tuesday.
The virus, first detected in the U.S. in 1987, causes difficult reproduction, limited weight gain and high mortality in pigs. To date, no vaccine has been effective, and the disease costs North American farmers more than $660 million annually.
"Once inside the pigs, PRRS needs some help to spread; it gets that help from a protein called CD163," said Randall Prather, MU distinguished professor of animal sciences. "We were able to breed a litter of pigs that do not produce this protein, and as a result, the virus doesn't spread. When we exposed the pigs to PRRS, they did not get sick and continued to gain weight normally."
For years, scientists have been trying to determine how the virus infected pigs and how to stop it.
Previously, researchers believed that the virus entered pigs by being inhaled into the lungs, where it attached to a protein known as sialoadhesin on the surface of white blood cells in the lungs. However, two years ago Prather's group showed that elimination of sialoadhesin had no effect on susceptibility to PRRS. A second protein, called CD163, was thought to "uncoat" the virus and allow it to infect the pigs. In their current study, Prather's team worked to stop the pigs from producing CD163.
"We edited the gene that makes the CD163 protein so the pigs could no longer produce it," said Kristin Whitworth, co-author on the study and a research scientist in MU's Division of Animal Sciences. "We then infected these pigs and control pigs; the pigs without CD163 never got sick. This discovery could have enormous implications for pig producers and the food industry throughout the world."
While the pigs that didn't produce CD163 didn't get sick, scientists also observed no other changes in their development compared to pigs that produce the protein.
The early-stage results of this research are promising. The University of Missouri has signed an exclusive global licensing deal for potential future commercialization of virus resistant pigs with the Genus, plc.
If the development stage is successful, MU said the commercial partner will seek any necessary approvals and registration from governments before a wider market release.
"At the end of our study, we had been able to make pigs that are resistant to an incurable, untreatable disease," said Kevin Wells, co-author of the study and assistant professor of animal sciences at MU. "This discovery could save the swine industry hundreds of millions of dollars every year. It also could have an impact on how we address other substantial diseases in other species."
In addition to Whitworth and Wells, Prather's research team included collaborators at Genus plc, and Kansas State University.
The study, "Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus," is being published in Nature Biotechnology this month.